CD114 Antibody (APC)

• Catalog Number: orb1952609
• Category: Antibodies
• Description: CD114 / G-CSFR (granulocyte colony-stimulating factor receptor, also known as CSF3R) is a type I transmembrane glycoprotein which upon binding of its ligand (G-CSF, granulocyte colony-stimulating factor) homodimerizes and activates signaling transduction to mediate cell proliferation, survival, and differentiation. It is expressed by granulocytes at all stages of their differentiation, as well as by monocytes, dendritic cells, and mature platelets. Among non-hematopoietic cells, it is expressed e.g. by endothelial cells, placenta, trophoblasts, and many tumor cell lines. This antigen is a target for stem cell mobilization for blood stem cell transplantation, for enhancing recovery of myelopoiesis following chemotherapy and in the treatment of patients with severe chronic neutropenia.
• Target: CD114
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: APC
• Reactivity: Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: CHO cells transfected with human CD114
• UniProt ID: Q99062
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Clone Number: LMM741
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: GCSFR, CSF3R, SCN7
• Note: For research use only
• Entrez: 1441
• Expiration Date: 12 months from date of receipt.

Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD114 (LMM741) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD114 (LMM741) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).