C5aR2 Antibody (APC)

• Catalog Number: orb1909789
• Category: Antibodies
• Description: C5aR2, also known as C5L2, is one of two receptors for C5a (anaphylatoxin). It is coexpressed with C5aR1 (CD88) in neutrophils, as well as e.g. in mast cells, astrocytes, or macrophages, and seems to have both pro-inflammatory and anti-inflammatory roles, depending on circumstances. Unlike CD88, C5aR2 is not coupled to G-protein, thus the modulatory role is more likely.
• Target: C5aR2
• Clonality: Monoclonal
• Isotype: Mouse IgG2a kappa
• Conjugation: APC
• Reactivity: Human, Primate
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: L1.2 cells transfected with human C5aR2
• UniProt ID: Q9P296
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Clone Number: 1D9-M12
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: C5L2, GPR77, GPF77
• Note: For research use only
• Entrez: 27202
• Expiration Date: 12 months from date of receipt.

Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human C5aR2 (1D9-M12) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human C5aR2 (1D9-M12) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).