beta2-Microglobulin Antibody (Biotin)

SKU: orb43655

Description

Anti-Hu beta2-Microglobulin Biotin

Images & Validation

• Tested Applications: ELISA, FC, ICC, IHC-P, IP, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-2 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes.Western blotting: Recommended dilution: 2 μg/ml; non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: B2M-01
• Immunogen: Purified human beta2-microglobulin
• Target: beta2-Microglobulin
• Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin

Storage & Handling

• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

B2M

Flow cytometry analysis (surface staining) of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) biotin.

Western blotting analysis of human β2-microglobulin using mouse monoclonal antibody B2M-01 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of biotinylated mouse monoclonal antibody followed by IRDye800-conjugated streptavidin. β2-microglobulin was detected around 12 kDa.

Quick Database Links

Gene Symbol

beta2-Microglobulin

UniProt

P61769

NCBI Gene ID

567