Anti-HSPB1 [SAIC-23C-35]

SKU: orb1089991

Description

Rabbit monoclonal antibody to HSPB1

Images & Validation

• Tested Applications: WB
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: SAIC-23C-35
• Immunogen: Peptide "VSLDVNHFAPDELTVK" derived from Heat shock protein beta-1, involved in stress resistance and actin organisation, conjugated to KLH.
• Target: HSPB1
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Heat shock protein beta-1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSP27; HSP28

Flow cytometry using the Anti-HSPB1 antibody SAIC-23C-35. Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of SAIC-23C-35 (orb1089991, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with anti-HSPB1 SAIC-23C-35. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-23C-35 (orb1089991) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089991, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-HSPB1 antibody SAIC-23C-35. HeLa(A) (0.03 µg/ml), MCF7(B) (0.001 µg/ml), Caco-2(C) (0.1 µg/ml) and A431(D) (0.03 µg/ml) cell lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-23C-35 (orb1089991), before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

HSPB1

UniProt

P04792