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Anti-HSPA7 Antibody

Catalog Number: orb1879466

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb1879466
CategoryAntibodies
DescriptionRabbit polyclonal antibody to HSPA7
ClonalityPolyclonal
Tested applicationsFC, IH, WB
ReactivityHuman
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the Central region of human HSPA7. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB (1/500 - 1/1000), IH (1/50 - 1/100), FC (1/10 - 1/30)
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
Entrez3311
UniProt IDP48741
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesHSP70B; Putative heat shock 70 kDa protein 7; Heat
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-HSPA7 Antibody

Western blot analysis of HSPA7 expression in 293 (A) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 70 kD)

Anti-HSPA7 Antibody

Immunohistochemical analysis of HSPA7 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-HSPA7 Antibody

Flow cytometric analysis of U2OS cells using Anti-HSPA7 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

  • HSPA7 Antibody [orb673122]

    ELISA,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    50 μg, 100 μg
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    ELISA,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    50 μl, 100 μl