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Anti-HSPA2 Rabbit Monoclonal Antibody

Catalog Number: orb548211

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Catalog Numberorb548211
CategoryAntibodies
DescriptionAnti-HSPA2 Rabbit Monoclonal Antibody
Species/HostRabbit
ClonalityMonoclonal
Clone NumberICB-8
Tested applicationsIHC, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenA synthesized peptide derived from human HSPA2
Antibody TypePrimary Antibody
ConcentrationActual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Form/AppearanceLiquid
ConjugationUnconjugated
MW106 kDa
UniProt IDP54652
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesHeat shock-related 70 kDa protein 2; Heat shock 70
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NoteFor research use only
Application notesWB 1:1000-1:2000IHC 1:50-1:200. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Expiration Date12 months from date of receipt.
Anti-HSPA2 Rabbit Monoclonal Antibody

Western blot analysis of HSPA2 expression in MCF-7 cell lysate (orb548211). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA2 monoclonal antibody (Catalog # orb548211) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for HSPA2.