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Anti-Hsp90 beta HSP90AB1 Rabbit Monoclonal Antibody

Catalog Number: orb547829

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Catalog Numberorb547829
CategoryAntibodies
DescriptionAnti-Hsp90 beta HSP90AB1 Rabbit Monoclonal Antibody
Species/HostRabbit
ClonalityMonoclonal
Clone NumberBAA-8
Tested applicationsFC, ICC, IF, IHC, IP, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenA synthesized peptide derived from human Hsp90 beta
Antibody TypePrimary Antibody
ConcentrationActual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Form/AppearanceLiquid
ConjugationUnconjugated
MW83264 Da
UniProt IDP08238
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesHeat shock protein HSP 90-beta; HSP 90; Heat shock
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NoteFor research use only
Application notesWB 1:500-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Expiration Date12 months from date of receipt.
Anti-Hsp90 beta HSP90AB1 Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human testis, using Hsp90 beta Antibody (orb547829). HSP90AB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSP90AB1 Antibody (orb547829) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

Anti-Hsp90 beta HSP90AB1 Rabbit Monoclonal Antibody

Western blot analysis of Hsp90 beta expression in (1) HeLa cell lysate; (2) Jurkat cell lysate (orb547829). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP90AB1 monoclonal antibody (Catalog # orb547829) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for HSP90AB1.

  • Anti-HSP90 alpha/beta Antibody [orb1474524]

    IF,  IH,  IP,  WB

    Human, Mouse, Rat

    Rabbit

    Monoclonal

    Unconjugated

    30 μl, 100 μl, 200 μl
  • Anti-Hsp90 beta HSP90AB1 Rabbit Monoclonal Antibody [orb547828]

    ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Monoclonal

    Unconjugated

    30 μl, 100 μl