Anti-HSP70 [cmhsp70.1 (C92F3B1)]

• Catalog Number: orb1821557
• Category: Antibodies
• Description: Anti-HSP70 [cmhsp70.1 (C92F3B1)]
• Target: HSP70
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: The original antibody was generated by grafting the CDRs of the mouse antibody on human framework regions. The parental mouse antibody was generated by immunizing mice with an SBD-derived synthetic peptide TKDNNLLGRFELSG (TKD peptide; aa 450–463) with complete Freud’s adjuvants.
• UniProt ID: P0DMV8
• Tested applications: ELISA, FC, IF, IHC, WB
• Clone Number: cmhsp70.1 (C92F3B1)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HSP70-1; HSP70.1; HSPA1A; HSX70; HSP72; mHsp70; He
• Note: For research use only

Flow cytometry using anti-HSP70 antibody cmhsp70.1 (C92F3B1). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of cmhsp70.1 (C92F3B1) (orb1821557, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with anti-HSP70 antibody cmhsp70.1 (C92F3B1). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of cmhsp70.1 (C92F3B1) (orb1821557) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1821557, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-HSP70 antibody cmhsp70.1 (C92F3B1). HEK293 (A) (0.0001 µg/ml), A431 (B) (0.0001 µg/ml), HeLa (C) (0.0001 µg/ml), MCF7 (D) (0.0001 µg/ml), A549 (E) (0.0001 µg/ml), and Jurkat (F) (0.001 µg/ml) cells lysate (35 µg protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of cmhsp70.1 (C92F3B1) (orb1821557) at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.