HSP27 Antibody

• Catalog Number: orb1474330
• Category: Antibodies
• Description: The HSP27 Antibody is suitable for IF, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant human Hsp27 protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Monkey
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Purified recombinant human Hsp27 protein fragments expressed in E.coli.
• UniProt ID: P04792
• Tested applications: IF, WB
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100)
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HSP27; HSP28; Heat shock protein beta-1; HspB1; 28
• Note: For research use only
• Entrez: 3315
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HSP27 expression in Hela (A), Hela (B), A431 (C), COS7 (D), Lncap (E), MCF7 (F) whole cell lysates. (Predicted band size: 23 kD; Observed band size: 27 kD)

Immunofluorescent analysis of HSP27 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.