Anti-HSF1 Antibody

• Catalog Number: orb1474526
• Category: Antibodies
• Description: Rabbit monoclonal antibody to HSF1
• Target: HSF1
• Clonality: Monoclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: A synthetic peptide of human HSF1
• UniProt ID: Q00613
• Tested applications: IH, IP, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50)
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HSTF1; Heat shock factor protein 1; HSF 1; Heat sh
• Note: For research use only
• Entrez: 3297

Western blot analysis of HSF1 expression in Hela (A), Jurkat (B) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 80 kD)

Immunohistochemical analysis of HSF1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.14). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.