HSF1 Rabbit Monoclonal Antibody

SKU: orb1474526

Description

The HSF1 Antibody is suitable for IHC, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against A synthetic peptide of human HSF1 .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human HSF1
• Target: HSF1
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1

Western blot analysis of HSF1 expression in Hela (A), Jurkat (B) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 80 kD)

Immunohistochemical analysis of HSF1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.14). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

HSF1

UniProt

Q00613

NCBI Gene ID

3297