Anti-hnRNP F Antibody

• Catalog Number: orb214043
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HNRNPF
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human hnRNP F. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HNRNPF
• Entrez: 3185, 98758
• UniProt ID: P52597, Q9Z2X1
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti HNRPF antibody, anti Heterogeneous nuclear ri
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of hnRNP F expression in HepG2 (A), Jurkat (B) whole cell lysates. (Predicted band size: 45 kD; Observed band size: 47 kD)

Immunofluorescent analysis of hnRNP F staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.