HMGB1 Antibody

SKU: orb1474610

Description

The HMGB1 Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against A synthetic peptide of human HMGB1 .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100)
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human HMGB1
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Disclaimer: For research use only

Alternative Names

HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1

Western blot analysis of HMGB1 expression in K562 (A), rat brain (B), C6 (C), NIH3T3 (D), Hela (E) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 25 kD)

Immunohistochemical analysis of HMGB1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.111). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

P63159, P09429, P63158

NCBI Gene ID

25459, 100862258, 15289, 3146