HLA-E Antibody (APC)

• Catalog Number: orb1881244
• Category: Antibodies
• Description: HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).
• Target: HLA-E
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: APC
• Reactivity: Human
• Concentration: 0.1 mg/ml
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: recombinant human HLA-E
• UniProt ID: P13747
• Tested applications: FC
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Clone Number: 3D12
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: HLA class I histocompatibility antigen, alpha chai
• Note: For research use only
• Entrez: 3133
• Expiration Date: 12 months from date of receipt.

Separation of human lymphocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-HLA-E (3D12) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-HLA-E (3D12) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).