HLA-DR1 (empty) Antibody (PE)

SKU: orb44259

Description

Mouse Monoclonal to HLA-DR1.

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: The antibody MEM-267 stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that express predominantly peptide loaded forms. Recommended dilution: 1-5 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: MEM-267
• Immunogen: Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.
• Target: HLA-DR1 (empty)
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 0.1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometry multicolor surface staining pattern of human peripheral whole blood stained using anti-human HLA-DR1-empty (MEM-267) PE antibody (concentration in sample 9 μg/ml) and anti-human HLA-DR (L243) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human HLA-DR1 (empty) positive HLA-DR positive cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA-DR1-empty (MEM-267) PE antibody (concentration in sample 9 μg/ml).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-DR1-empty (MEM-267) PE antibody (concentration in sample 9 μg/ml).

Quick Database Links

Gene Symbol

HLA-DR1 (empty)

NCBI Gene ID

35