HLA-DR Antibody

SKU: orb154429

Description

Mouse monoclonal antibody to HLA-DR which belongs to the MHC class II family. It localized in endoplasmic reticulum membrane, golgi apparatus. It binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC). HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4+ T lymphocytes.

Images & Validation

• Tested Applications: FA, FC, ICC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Canine, Human, Primate
• Application Notes:
  Functional application: Blocking. Flow cytometry: Recommended dilution: 1-4 µg/ml

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a kappa
• Clone No.: L243
• Immunogen: Human B lymphocytes
• Target: HLA-DR
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Separation of human HLA-DR positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA-DR (L243) purified antibody (concentration in sample 0.3 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-DR (L243) purified antibody (concentration in sample 0.3 μg/ml) GAM APC.

Quick Database Links

Gene Symbol

HLA-DR

NCBI Gene ID

35