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Item 1 of 2
Anti-HIWI1 Antibody
Catalog Number: orb340827
| Catalog Number | orb340827 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to PIWIL1 |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IP, WB |
| Reactivity | Human, Mouse, Rat |
| Immunogen | Recombinant full length protein of human HIWI1 |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:500-1:2000, IF/ICC: 1:20-1:100, IP: 1:20-1:100 |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Conjugation | Unconjugated |
| Target | PIWIL1 |
| Entrez | 9271, 57749 |
| UniProt ID | Q96J94, Q9JMB7 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Alternative names | HIWI; Piwi-like protein 1 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of HIWI1 expression in HepG2 (A), SKOV3 (B), mouse testis (C), rat testis (D) whole cell lysates. (Predicted band size: 95; 98 kD; Observed band size: 102; 110 kD)
Immunofluorescent analysis of HIWI1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
