Histone H4 Rabbit Polyclonal Antibody

SKU: orb224159

Description

The Histone H4 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1:500-1:1000, IHC-P: 1:100-1:200, IF/ICC: 1:100-1:500
• Reactivity: Bovine, Gallus, Human, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H4. The exact sequence is proprietary.
• Target: HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; HIST4H4
• Molecular Weight: 11 kDa
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H4/A; H4FA; H4/I; H4FI; H4/G; H4FG; H4/B; H4FB; H4/J; H4FJ; H4/C; H4FC; H4/H; H4FH; H4/M; H4FM; H4/E; H4FE; H4/D; H4FD; H4/K; H4FK; H4/N; H4F2; H4FN; HIST2H4; H4/O; H4FO; Histone H4

Immunofluorescent analysis of Histone H4 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Immunohistochemical analysis of paraffin-embedded human breast cancer using Histone H4 Antibody orb224159 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded human lung cancer using Histone H4 Antibody orb224159 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat large intestine using Histone H4 Antibody orb224159 with dilution at 1:100.

Western blot analysis of mouse lung (1), mouse liver (2), rat liver (3), mouse kidney (4) using Histone H4 Antibody orb224159 with dilution at 1:1000.

Quick Database Links

Gene Symbol

HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; HIST4H4

UniProt

P62806, P62805, P62804

NCBI Gene ID

295277, 8365, 100912290, 102641229, 8370, 319158, 8368, 100041230, 319155, 100912418, 100912564, 8367, 319161, 8294, 8360, 102557184, 8363, 102548682, 319156, 69386, 502913, 97122, 100360950, 319159, 8364, 8361, 680097, 319160, 319157, 64627, 326619, 326620, 291152, 8359, 8366, 8362, 554313, 320332, 121504, 102551184, 500351