Anti-Histone H3 (TriMethyl-K4) Antibody

• Catalog Number: orb1289911
• Category: Antibodies
• Description: Rabbit Polyclonal antibody to Histone H3
• Target: HIST1H3A; HIST1H3; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; H3F3A; H3F3B; H3F3C
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat, Zebrafish
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic Tri-methylated peptide corresponding to residues surrounding K4 of human Histone H3 protein. The exact sequence is proprietary.
• UniProt ID: P84243, Q71DI3, P68431, Q6NXT2
• Tested applications: IH, WB
• Dilution range: WB: 1:500-1:1000, IH: 1:50-1:200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HI
• Note: For research use only
• Entrez: 3020, 126961, 8355, 8968, 8353, 8357, 440093, 8358, 8352, 3021, 8356, 8350, 333932, 8354, 653604, 8351

Western blot analysis of Histone H3 (TriMethyl-K4) expression in mouse muscle (A), rat lung (B), rat muscle (C) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

Immunohistochemical analysis of Histone H3 (TriMethyl-K4) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Direct ELISA antibody dose-response curve using Anti-Histone H3 (TriMethyl-K4) Antibody. Antigen (methyl-peptide and non-methyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.