Anti-Histone H3 (TriMethyl-K27) Antibody

• Catalog Number: orb412098
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H3
• Target: HIST3H3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic Tri-methylated peptide corresponding to residues surrounding K27 of human Histone H3 protein. The exact sequence is proprietary.
• UniProt ID: Q16695
• Tested applications: ChIP, IF, IH, IP, WB
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200, IP: 1:50-100, ChIP: 1:100-500
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-H3FT antibody, anti-Histone H3.1t antibody, a
• Note: For research use only
• Entrez: 8290

Western blot analysis of Histone H3 (TriMethyl-K27) expression in Hela (A) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

Immunofluorescent analysis of Histone H3 (TriMethyl-K27) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.