Anti-Histone H3 (TriMethyl-K27) Antibody

• Catalog Number: orb1474467
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Histone H3 (TriMethyl-K27)
• Target: H3C1
• Clonality: Monoclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic Tri-methylated peptide corresponding to residues surrounding K27 of human Histone H3 protein. The exact sequence is proprietary.
• UniProt ID: Q6LED0, P68431
• Tested applications: ChIP, IF, IH, IP, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50), ChIP (1/10 - 1/50)
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H3FA; H3FL; H3FC; H3FB; H3FD; H3FI; H3FH; H3FK; H3
• Note: For research use only
• Entrez: 682330, 8350, 291159

Western blot analysis of Histone H3 (TriMethyl-K27) expression in C6 (A), Jurkat (B) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 15 kD)

Immunohistochemical analysis of Histone H3 (TriMethyl-K27) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.90). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Histone H3 (TriMethyl-K27) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.