Anti-Histone H3 (TriMethyl-K14) Antibody

• Catalog Number: orb334721
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H3 (TriMethyl-K14)
• Target: HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; H3F3A; H3F3B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic Tri-methylated peptide corresponding to residues surrounding K14 of human Histone H3 protein. The exact sequence is proprietary.
• UniProt ID: Q71DI3, P68431, Q6LED0, P84243, P84245
• Tested applications: IH, WB
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:200-1:500
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti HIST1H3A antibody, anti H3FA antibody, anti H
• Note: For research use only
• Entrez: 100361558, 8353, 8968, 8351, 8357, 8350, 291159, 8352, 8355, 8356, 8354, 8358

Western blot analysis of Histone H3 (TriMethyl-K14) expression in Hela (A), rat testis (B) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

Immunohistochemical analysis of Histone H3 (TriMethyl-K14) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.