Histone H3 (DiMethyl-K4) Antibody

SKU: orb1289912

Description

The Histone H3 (DiMethyl-K4) Antibody is suitable for WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic Di-methylated peptide corresponding to residues surrounding K4 of human Histone H3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

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Tested Applications	WB
Dilution range	WB: 1:500-1:1000
Reactivity	Bovine, Gallus, Human, Mouse, Porcine, Rat, Zebrafish

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH-conjugated synthetic Di-methylated peptide corresponding to residues surrounding K4 of human Histone H3 protein. The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Disclaimer	For research use only

Alternative Names

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HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3; H3F3C; Histone H3.3C; Histone H3.5

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Western blot analysis of Histone H3 (DiMethyl-K4) expression in U2OS (A), DLD (B) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

Direct ELISA antibody dose-response curve using Anti-Histone H3 (DiMethyl-K4) Antibody. Antigen (methyl-peptide and non-methyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Quick Database Links

UniProt

P84243, Q71DI3, Q6NXT2, P68431

NCBI Gene ID

8353, 8356, 8357, 3020, 440093, 3021, 8355, 126961, 8351, 8352, 8354, 333932, 8968, 8350, 653604, 8358

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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