Histone H3 Antibody

• Catalog Number: orb323170
• Category: Antibodies
• Description: The Histone H3 Antibody is suitable for IF, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant protein corresponding to human Histone H3. Purification: Affinity chromatography
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat, Yeast
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: Affinity chromatography
• Immunogen: Recombinant protein corresponding to human Histone H3.
• UniProt ID: P84228, Q71DI3, P68431, P68433, P84243, P84244, Q6LED0, P84245
• Tested applications: IF, IP, WB
• Dilution range: WB: 1:2000-1:5000, IF/ICC: 1:100-1:500, IP: 1:50-1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HI
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 8353, 8356, 8357, 319152, 8355, 8351, 8352, 8354, 15077, 100361558, 8968, 8350, 15078, 291159, 8358
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone H3 expression in Hela (A), Raw264.7 (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

Immunofluorescent analysis of Histone H3 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Immunoprecipitation of Histone H3 from 0.5 mg Hela whole cell extract lysate, using Anti-Histone H3 Antibody.