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| Catalog Number | orb1289782 |
|---|---|
| Category | Antibodies |
| Description | The Histone H3 (Acetyl-K36) Antibody is suitable for WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K36 of human Histone H3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Bovine, Gallus, Human, Mouse, Porcine, Rat, Zebrafish |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Immunogen | KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K36 of human Histone H3 protein. The exact sequence is proprietary. |
| UniProt ID | P84228, Q71DI3, Q6NXT2, P68431, P68433, P84243, P84244, P02301, Q6LED0, P84245 |
| Tested applications | WB |
| Dilution range | WB: 1:500-1:1000 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HI Read more... |
| Research Area | Epigenetics |
| Note | For research use only |
| Entrez | 8355, 15077, 291159, 8356, 8357, 3020, 3021, 8354, 625328, 8968, 653604, 8353, 440093, 126961, 8351, 100361558, 8350, 15078, 8358, 319152, 8352, 333932 |

Western blot analysis of Histone H3 (Acetyl-K36) expression in SHSY5Y (A), EC9706 (B), BV2 (C) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

Direct ELISA antibody dose-response curve using Anti-Histone H3 (Acetyl-K36) Antibody. Antigen (Acetyl-peptide and non-acetyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Gallus, Human, Mouse, Porcine, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Other, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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