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Anti-Histone H3 (Acetyl-K27) Antibody

Catalog Number: orb1474466

DispatchUsually dispatched within 5-10 working days
$ 190.00
Catalog Numberorb1474466
CategoryAntibodies
DescriptionRabbit monoclonal antibody to Histone H3 (Acetyl-K27)
Species/HostRabbit
ClonalityMonoclonal
Tested applicationsChIP, IF, IH, IP, WB
ReactivityHuman
ImmunogenKLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K27 of human Histone H3 protein. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50), ChIP (1/10 - 1/50)
Form/AppearanceLiquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
ConjugationUnconjugated
TargetH3C1
Entrez8350
UniProt IDP68431
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
Alternative namesH3FA; H3FL; H3FC; H3FB; H3FD; H3FI; H3FH; H3FK; H3
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-Histone H3 (Acetyl-K27) Antibody

Western blot analysis of Histone H3 (Acetyl-K27) expression in Hela (A), A549 (B), HL60 (C) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 15 kD)

Anti-Histone H3 (Acetyl-K27) Antibody

Immunohistochemical analysis of Histone H3 (Acetyl-K27) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.91). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Histone H3 (Acetyl-K27) Antibody

Immunofluorescent analysis of Histone H3 (Acetyl-K27) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

  • Anti-Histone H3 (Acetyl-K27) Antibody [orb256598]

    IF,  IH,  WB

    Human, Mouse, Porcine, Primate, Rabbit, Rat, Zebrafish

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 30 μl
  • Anti-Histone H3 (Acetyl-K27) Antibody [orb412573]

    IF,  IH,  IP,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 200 μl, 100 μl
  • Anti-Histone H3 (Acetyl-K27) Antibody [orb334689]

    IH,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 30 μl
  • Anti-Histone H3 (acetyl K27) Rabbit Monoclonal Antibody [orb866691]

    ChIP,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Monoclonal

    Unconjugated

    30 μl, 100 μl