Histone H3 (Acetyl-K14) Antibody

• Catalog Number: orb1474786
• Category: Antibodies
• Description: The Histone H3 (Acetyl-K14) Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K14 of human Histone H3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Zebrafish
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K14 of human Histone H3 protein. The exact sequence is proprietary.
• UniProt ID: P84243, Q71DI3, P68431
• Tested applications: IF, WB
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HI
• Note: For research use only
• Entrez: 8353, 8356, 8357, 3020, 3021, 8355, 126961, 8351, 8352, 8354, 333932, 8968, 8350, 653604, 8358

Western blot analysis of Histone H3 (Acetyl-K14) expression in zebrafish (A) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 15 kD)

Immunofluorescent analysis of Histone H3 (Acetyl-K14) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.