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Description
Research Area
Epigenetics & Chromatin
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB: 1:1000-1:3000, IHC-P: 1:200-1:500 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence of human Histone H2B. The exact sequence is proprietary. |
| Target | HIST1H2BB |
| Purification | Affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f
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Western blot analysis of Histone H2B expression in Hela (A), NIH3T3 (B), Raw264.7 (C), rat brain (D), rat kidney (E) whole cell lysates. (Predicted band size: 13 kD; Observed band size: 14 kD)
Immunohistochemical analysis of Histone H2B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
Histone H2B Mouse Monoclonal Antibody (orb323154)
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