Histone H2B (Acetyl-K15) Rabbit Polyclonal Antibody

SKU: orb256582

Description

The Histone H2B (Acetyl-K15) Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K15 of human Histone H2B protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
• Reactivity: Bovine, Canine, Human, Monkey, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K15 of human Histone H2B protein. The exact sequence is proprietary.
• Target: H2BFS
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Histone H2B type F-S; Histone H2B.s; H2B/s

Western blot analysis of Histone H2B (Acetyl-K15) expression in rat thymus (A), mouse spleen (B), H9C2 (C), Raw264.7 (D), HepG2 (E), HuT78 (F), SHSY5Y (G) whole cell lysates. (Predicted band size: 13 kD; Observed band size: 14 kD)

Immunohistochemical analysis of Histone H2B (Acetyl-K15) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Histone H2B (Acetyl-K15) staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

H2BFS

UniProt

P57053

NCBI Gene ID

102724334