Histone H2B (Acetyl-K125) Rabbit Polyclonal Antibody

SKU: orb1289784

Description

The Histone H2B (Acetyl-K125) Antibody is suitable for WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K125 of human Histone H2B protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:500-1:1000
• Reactivity: Bovine, Gallus, Human, Mouse, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K125 of human Histone H2B protein. The exact sequence is proprietary.
• Target: HIST1H2BC
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HIST1H2BA; TSH2B; Histone H2B type 1-A; Histone H2B testis; Testis-specific histone H2B; HIST1H2BB; H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f; HIST1H2BC; H2BFL; HIST1H2BE; H2BFH; HIST1H2BF; H2BFG; HIST1H2BG; H2BFA; HIST1H2BI; H2BFK; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l

Western blot analysis of Histone H2B (Acetyl-K125) expression in SP20 (A), SHSY5Y (B), C6 (C), PC3 (D) whole cell lysates. (Predicted band size: 14; 13 kD; Observed band size: 17 kD)

Direct ELISA antibody dose-response curve using Anti-Histone H2B (Acetyl-K125) Antibody. Antigen (Acetyl-peptide and non-acetyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Quick Database Links

Gene Symbol

HIST1H2BC

UniProt

Q64475, Q6ZWY9, P33778, Q00729, P70696, P62807, Q96A08

NCBI Gene ID

319179, 8347, 8339, 24829, 255626, 3018, 8346, 8344, 8343, 3017, 319177, 319178