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Anti-Histone H1.0 H1F0 Rabbit Monoclonal Antibody

Catalog Number: orb548466

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb548466
CategoryAntibodies
DescriptionAnti-Histone H1.0 H1F0 Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
ClonalityMonoclonal
Species/HostRabbit
IsotypeRabbit IgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid
ConcentrationActual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
ImmunogenA synthesized peptide derived from human Histone H1.0
UniProt IDP07305
MW23 kDa
Tested applicationsICC, IF, IHC, WB
Application notesWB 1:1000-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Antibody TypePrimary Antibody
Clone NumberHAG-8
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesHistone H1.0; Histone H1'; Histone H1 (0); Histone
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NoteFor research use only
Anti-Histone H1.0 H1F0 Rabbit Monoclonal Antibody

Western blot analysis of Histone H10 expression in Human kidney lysate (orb548466). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H1F0 monoclonal antibody (Catalog # orb548466) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for H1F0.