Histone Deacetylase 5/9 (Phospho-S259/220) Antibody

SKU: orb393071

Description

The Histone Deacetylase 5/9 (Phospho-S259/220) Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S259/220 of human Histone Deacetylase 5/9 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB: 1:500:1000, IHC-P: 1:100:200
• Reactivity: Bovine, Canine, Gallus, Human, Mouse, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S259/220 of human Histone Deacetylase 5/9 protein. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

HDAC5; KIAA0600; Histone deacetylase 5; HD5; Antigen NY-CO-9; HDAC9; HDAC7; HDAC7B; HDRP; KIAA0744; MITR; Histone deacetylase 9; HD9; Histone deacetylase 7B; HD7; HD7b; Histone deacetylase-related protein; MEF2-interacting transcription repressor MITR

Western blot analysis of Histone Deacetylase 5/9 (Phospho-S259/220) expression in PC3 (A), HeLa (B), NIH3T3 (C) whole cell lysates. (Predicted band size: 121; 111 kD; Observed band size: 124 kD)

Immunohistochemical analysis of Histone Deacetylase 5/9 (Phospho-S259/220) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

Q9UKV0, Q9UQL6, Q99N13, Q9Z2V6

NCBI Gene ID

79221, 9734, 10014