Histamine H2 Receptor Antibody

SKU: orb214058

Description

The Histamine H2 Receptor Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Histamine H2 Receptor. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Histamine H2 Receptor. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

Histamine H2 receptor; H2R; HH2R; Gastric receptor I

Western blot analysis of Histamine H2 Receptor expression in mouse kidney (A), rat liver (B) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 40 kD)

Immunofluorescent analysis of Histamine H2 Receptor staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

UniProt

P25021, P25102, P97292

NCBI Gene ID

15466, 3274, 25461