HIP1 Monoclonal Antibody

SKU: orb547985

Description

Anti-HIP1 Monoclonal Antibody. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Images & Validation

• Tested Applications: ICC, IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB 1:500-2000ICC/IF 1:50-200IP 1:30. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: AFG-8
• Immunogen: A synthesized peptide derived from human HIP1 Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Enhances androgen receptor (AR) -mediated transcription.
• Molecular Weight: 116 kDa
• Purification: Affinity-chromatography

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Disclaimer: For research use only

Alternative Names

T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8a; CD8A; MAL

Western blot analysis of HIP1 using anti-HIP1 antibody (orb547985). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HeLa whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HIP1 antigen affinity purified monoclonal antibody (Catalog # orb547985) at 1: 1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90502) with Tanon 5200 system. A specific band was detected for HIP1 at approximately 116 kDa. The expected band size for HIP1 is at 116 kDa.

Quick Database Links

UniProt

O00291