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Anti-HIF-1 beta ARNT Rabbit Monoclonal Antibody
Catalog Number: orb547989
| Catalog Number | orb547989 |
|---|---|
| Category | Antibodies |
| Description | Anti-HIF-1 beta ARNT Rabbit Monoclonal Antibody. Tested in WB, IHC applications. This antibody reacts with Human. |
| Species/Host | Rabbit |
| Clonality | Monoclonal |
| Clone Number | EAD-1 |
| Tested applications | IHC, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Immunogen | A synthesized peptide derived from human HIF-1 beta |
| Antibody Type | Primary Antibody |
| Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 16 kDa |
| UniProt ID | P27540 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Aryl hydrocarbon receptor nuclear translocator; AR Read more... |
| Note | For research use only |
| Application notes | WB 1:500-1:2000IHC 1:500-1:2000. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded human colon, using HIF-1 beta Antibody (orb547989) ARNT was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ARNT Antibody (orb547989) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.
Western blot analysis of HIF-1 beta expression in Human fetal kidney lysate (orb547989). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARNT monoclonal antibody (Catalog # orb547989) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for ARNT.
