HFM1 Antibody

• Catalog Number: orb412805
• Category: Antibodies
• Description: The HFM1 Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human HFM1 .Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human HFM1
• UniProt ID: A2PYH4
• Tested applications: IHC, WB
• Dilution range: WB: 1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SEC3D1; Probable ATP-dependent DNA helicase HFM1;
• Note: For research use only
• Entrez: 164045

Western blot analysis of HFM1 expression in K562 (A) whole cell lysates. (Predicted band size: 62; 162 kD; Observed band size: 130 kD)

Immunohistochemical analysis of HFM1 staining in rat testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.