Anti-Hexokinase 2 Antibody

• Catalog Number: orb1474527
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Hexokinase 2
• Target: HK2
• Clonality: Monoclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Hamster, Human, Rat
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant protein of human Hexokinase II
• UniProt ID: P27881, P52789
• Tested applications: IH, IP, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50)
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Hexokinase-2; Hexokinase type II; HK II; Muscle fo
• Note: For research use only
• Entrez: 3099, 25059

Western blot analysis of Hexokinase 2 expression in rat skeletal muscle (A), Hela (B), CHOK1 (C) whole cell lysates. (Predicted band size: 102 kD; Observed band size: 102 kD)

Immunohistochemical analysis of Hexokinase 2 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.13). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.