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Item 1 of 2
Item 1 of 2
| Catalog Number | orb315778 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to HER2 |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IH, WB |
| Reactivity | Human, Mouse, Rat |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1248 of human HER2 protein. The exact sequence is proprietary. |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1-500:1000, IHC-P: 1-100:200, IF/ICC: 1-100:500, IP: 1-10:100 |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Conjugation | Unconjugated |
| Target | ERBB2 |
| Entrez | 13866, 2064 |
| UniProt ID | P04626, P06494, P70424 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Alternative names | anti-CD340 antibody, anti- ERBB2 antibody, anti- H Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
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A Razavirad, S Rismanchi, P Mortazavi, A Muhammadnejad Canine Mammary Tumors as a Potential Model for Human Breast Cancer in Comparative Oncology Veterinary Medicine International, (2024)
Applications
IHC
Western blot analysis of HER2 (Phospho-Y1248) expression in MCF7 (A), EC9706 (B) whole cell lysates. (Predicted band size: 137 kD; Observed band size: 185 kD)
Immunohistochemical analysis of HER2 (Phospho-Y1248) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
