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Catalog Number | orb222727 |
---|---|
Category | Antibodies |
Description | Hamster monoclonal antibody against Helios conjugated to PE |
Clonality | Monoclonal |
Clone Number | 22F6 |
Tested applications | FC |
Reactivity | Human, Mouse |
Isotype | Armenian Hamster IgG |
Immunogen | Peptide coresponding to the amino acids 51-107 of Helios |
Antibody Type | Primary Antibody |
Purity | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | PE |
Target | Helios |
Entrez | 22807 |
UniProt ID | Q9UKS7 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-ANF1A2 antibody, anti-IKZF2 antibody Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining. |
Expiration Date | 12 months from date of receipt. |
Human peripheral blood lymphocytes were stained with anti-CD4 Pacific BlueTM, fixed and permeabilized with Treg Detection Kit (Miltenyi), and then intracellulary stained with anti-Helios PE (clone 22F6).