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Anti-HCCR-1 Antibody

Catalog Number: orb340824

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb340824
CategoryAntibodies
DescriptionRabbit polyclonal antibody to LETMD1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant full length protein of human HCCR-1
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:50-1:200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetLETMD1
Entrez25875, 68614
UniProt IDQ6P1Q0, Q924L1
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesLETM1 domain-containing protein 1; Cervical cancer
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-HCCR-1 Antibody

Western blot analysis of HCCR-1 expression in mouse kidney (A), mouse liver (B) whole cell lysates. (Predicted band size: 11; 17; 23; 35; 41; 43 kD; Observed band size: 41 kD)

Anti-HCCR-1 Antibody

Immunohistochemical analysis of HCCR-1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-HCCR-1 Antibody

Immunofluorescent analysis of HCCR-1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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    Polyclonal

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    100 μl
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    IF,  IHC,  WB

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    Rabbit

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