Anti-HAND1 Antibody

• Catalog Number: orb256583
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HAND1
• Target: HAND1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Porcine, Rabbit, Rat, Sheep
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human HAND1. The exact sequence is proprietary.
• UniProt ID: O96004, Q64279, P97832
• Tested applications: IF, WB
• Dilution range: WB: 1:500-1000, IF/ICC: 1:100-500
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti BHLHA27 antibody, anti EHAND antibody, anti H
• Note: For research use only
• Entrez: 9421, 15110, 59112

Western blot analysis of HAND1 expression in HeLa (A), H9C2 (B) whole cell lysates. (Predicted band size: 23 kD; Observed band size: 25 kD)

Immunofluorescent analysis of HAND1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).