HACE1 Antibody

• Catalog Number: orb666862
• Category: Antibodies
• Description: The HACE1 Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide of human HACE1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide of human HACE1. The exact sequence is proprietary.
• UniProt ID: Q3U0D9, D3ZBM7, Q8IYU2
• Tested applications: IHC, WB
• Dilution range: WB: 1-500-2000, IHC-P: 1-50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KIAA1320; E3 ubiquitin-protein ligase HACE1; HECT
• Research Area: Neuroscience
• Note: For research use only
• Entrez: 361866, 209462, 57531

Western blot analysis of HACE1 expression in HeLa (A), mouse lung (B), mouse spleen (C) whole cell lysates. (Predicted band size: 35; 63; 77; 102 kD; Observed band size: 102 kD)

Immunohistochemical analysis of HACE1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.