Anti-GSK3 beta (Phospho-S9) Antibody

• Catalog Number: orb393067
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GSK3B (Phospho-S9)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rat, Sheep, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S9 of human GSK3 beta protein. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500:1000, IHC-P: 1:100:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GSK3B
• Entrez: 56637, 84027, 2932
• UniProt ID: P49841, Q9WV60, P18266
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Glycogen synthase kinase-3 beta; GSK-3 beta; Serin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Rao, Chinthalapally V. et al. GSK3-ARC/Arg3.1 and GSK3-Wnt signaling axes trigger amyloid-β accumulation and neuroinflammation in middle-aged Shugoshin 1 mice Aging Cell, 19, e13221 (2020)

PubMed: 32857910

Western blot analysis of GSK3 beta (Phospho-S9) expression in MCF7-insulin-5min (A), MCF7 (B), Hela-insulin-15min (C) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46 kD)

Immunohistochemical analysis of GSK3 beta (Phospho-S9) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.