Anti-GRP78 Antibody

• Catalog Number: orb1474230
• Category: Antibodies
• Description: Mouse monoclonal antibody to GRP78
• Target: HSPA5
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Immunogen: Purified recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E.coli.
• UniProt ID: P11021, P06761
• Tested applications: IF, IH, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100)
• Antibody Type: Primary Antibody
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GRP78; 78 kDa glucose-regulated protein; GRP-78; E
• Note: For research use only
• Entrez: 3309, 25617

Western blot analysis of GRP78 expression in Hela (A), C6 (B), Lncap (C), MDAMB468 (D) whole cell lysates. (Predicted band size: 72 kD; Observed band size: 78 kD)

Immunohistochemical analysis of GRP78 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.68). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of GRP78 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.