Anti-GRB2 [SAIC-19D-1F4]

• Catalog Number: orb613818
• Category: Antibodies
• Description: Mouse monoclonal antibody to GRB2
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: SAIC-19D-1F4
• Tested applications: WB
• Reactivity: Human
• Isotype: Rabbit IgG1
• Immunogen: Peptide "FGNDVQHFK", derived from the Growth factor receptor-bound protein 2, conjugated to KLH.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: GRB2
• UniProt ID: P62993
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Growth factor receptor-bound protein 2, ASH
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed HeLa cells with anti-GRB2 antibody SAIC-19D-1F4. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of SAIC-19D-1F4 at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing nuclear staining. The nuclear DNA stain is DAPI (blue).

Western Blot using anti-GRB2 antibody SAIC-19D-1F4 Human spleen lysate samples (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of SAIC-19D-1F4 at 0.001 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for GRB2 is 25.2kDa.