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Anti-GPR52 Antibody

Catalog Number: orb214885

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb214885
CategoryAntibodies
DescriptionRabbit polyclonal antibody to GPR52
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPR52. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000, IP: 1-10-1-100
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetGPR52
Entrez9293
UniProt IDQ9Y2T5
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti Probable G-protein coupled receptor 52 antibo
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-GPR52 Antibody

Western blot analysis of GPR52 expression in BV2 (A), PC12 (B), A549 (C), HepG2 (D), HCT116 (E) whole cell lysates. (Predicted band size: 41 kD; Observed band size: 50 kD)

Anti-GPR52 Antibody

Immunofluorescent analysis of GPR52 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

  • GPR52 Antibody [orb675593]

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  • Anti-GPR52 Antibody [orb1423314]

    IP,  WB

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    Polyclonal

    Unconjugated

    100 μl, 50 μl
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    Synthetic

    5 mg, 1 mg