Anti-GPR18 Antibody

• Catalog Number: orb213995
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GPR18 also known as NAGly receptor whose expression can be detected in the choroid plexus and is highly expressive in the lungs and testis. It is found in the cytoplasmic vesicle. This protein functions as a receptor for endocannabinoid N-arachidonyl glycine (NAGly). It plays a role in hypotensive responses mediating reduction in intraocular and blood pressure and also mediates NAGly-induced process of reorganization of actin filaments and induction of acrosomal exocytosis.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR18. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GPR18
• Entrez: 110168, 679957, 2841
• UniProt ID: Q14330, Q8K1Z6, A1A5S3
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-GPCRW antibody, anti-N-arachidonyl glycine re
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GPR18 expression in Jurkat (A) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 38 kD)

Immunofluorescent analysis of GPR18 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).