GPR18 Antibody

SKU: orb213995

Description

The GPR18 Antibody is suitable for FC, IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR18. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR18. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

GPCRW; N-arachidonyl glycine receptor; NAGly receptor; G-protein coupled receptor 18

Western blot analysis of GPR18 expression in Jurkat (A) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 38 kD)

Immunofluorescent analysis of GPR18 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

UniProt

Q14330, A1A5S3, Q8K1Z6

NCBI Gene ID

679957, 2841, 110168