GPR176 Antibody

• Catalog Number: orb215050
• Category: Antibodies
• Description: The GPR176 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPR176. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPR176. The exact sequence is proprietary.
• UniProt ID: Q80WT4, Q64017, Q14439
• Tested applications: IF, WB
• Dilution range: WB: 1-500-1-1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Probable G-protein coupled receptor 176; HB-954
• Research Area: Epigenetics, GPCR, Neuroscience, Signaling Pathway
• Note: For research use only
• Entrez: 381413, 117257, 11245

Western blot analysis of GPR176 expression in HEK293T (A), Hela (B), U2OS (C), mouse testis (D), mouse heart (E), rat testis (F), rat heart (G) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 57 kD)

Immunofluorescent analysis of GPR176 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.