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Anti-GPR13 Antibody

Catalog Number: orb234864

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb234864
CategoryAntibodies
DescriptionRabbit polyclonal antibody to GPR13
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPR13. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetCX3CR1
Entrez171056, 13051, 1524
UniProt IDQ9Z0D9, P35411, P49238
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti CMKBRL1 antibody, anti GPR13 antibody, anti C
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-GPR13 Antibody

Western blot analysis of GPR13 expression in HepG2 (A), mouse spleen (B), rat spleen (C), DLD (D) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 50 kD)

Anti-GPR13 Antibody

Immunohistochemical analysis of GPR13 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-GPR13 Antibody

Immunofluorescent analysis of GPR13 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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