GPR115 Rabbit Polyclonal Antibody

SKU: orb515240

Description

The GPR115 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPR115. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, GPCR, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500-1000, IF/ICC: 1:100-500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPR115. The exact sequence is proprietary.
• Target: GPR115
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PGR18; Probable G-protein coupled receptor 115; G-protein coupled receptor PGR18

Western blot analysis of GPR115 expression in BV2 (A), PC12 (B), A549 (C), HepG2 (D) whole cell lysates. (Predicted band size: 77 kD; Observed band size: 110 kD)

Immunofluorescent analysis of GPR115 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

GPR115

UniProt

Q9D2L6, Q8IZF3

NCBI Gene ID

78249, 221393