GPC3/Glypican-3 Antibody

• Catalog Number: orb1806346
• Category: Antibodies
• Description: Anti-GPC3 / Glypican-3 Reference Antibody is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
• Target: GPC3 / Glypican-3
• Clonality: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/mL
• Buffer/Preservatives: 100 mM Proline/Acetic acid 20mM Arginine pH 5.0
• Purity: >95%
• Purification: Protein A
• UniProt ID: P51654
• MW: 145.5 kDa
• Tested applications: ELISA, FA, FACS, In vivo
• Expression System: CHO
• Endotoxins: <0.001 EU/ug
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab)is more than 95% ;determined by SEC-HPLC.

Immobilized human GPC3; His tag at 2 μg/mL can bind Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab);EC50=0.008741μg/mL.

Human GPC3 CHOS cells were stained with Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC210=2.142 nM.

The endocytosis ratio codrituzumab by HuH-7 increased with the increase of antibody concentration; and the Internalization Rate (%) reached 40%at antibody concentration of 1 nM.

Anti-GPC3 / Glypican-3 Reference Antibody (Codrituzumab)-induced ADCC activity was evaluated using human GPC3 CHOS Cell. The max Lysis rate was approximately 30%.